Coding
BFRM52H

Part:BBa_K1438001:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-06)


Bacterioferritin (BFR) M52H heme-deletion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106


Design Notes

The M52H Mutagenesis leads to a deletion of the BFR heme residue. As a result, BFR will have a reduced iron release rate and keep its iron content inside of the protein scaffold.

Source

Escherichia coli Nissle 1917

References